Optimum chance phylogenetic relationships of control area haplotypes produced from market bought eyeball examples and sequences acquired from understood people.
Phylogenetic relationships had been identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony length = 141). Help values mentioned above nodes are Bayesian probabilities centered on 5,000,000 resamples, maximum chance bootstrap help values according to 1,000 bootstrap replicates, and maximum parsimony bootstrap help values according to 2,000 bootstrap replicates. Help values are shown limited to clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and also for the monophyly of Sotalia. Branch lengths are proportional to likelihood that is maximum divergence; scale suggests portion of series divergence. Haplotypes 1–14, highlighted in bold, were present in our research and correspond to GenBank accession #EU022531–EU022544.
To help expand confirm the relationships of this unknown sequences, we performed a nonphylogenetic cladistic analysis. We constructed a matrix of autapomorphies for Sotalia fluviatilis, Sotalia guianensis, Inia g. Geoffrensis, Inia g. Humboltiana, and Inia g. Boliviensis predicated on available sequence information in Genbank and our guide people, and performed a hot latina nude population aggregation analysis ( Davis and Nixon 1992 ).
All eyeball?derived sequences decided by BLAST to be of cetacean origin provided species?specific autapomorphic character states indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis. The control region and Cytochrome b information sets have two and six diagnostic internet sites, respectively, that assign all eyeball that is cetacean to Sotalia guianensis and unambiguously differentiate it from Sotalia fluviatilis and Inia spp. ( dining Table 1; online Appendix S1, S2).
| Control area | BEL | MAO | PVH | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Variable position | 39 | 136 | 139 | 160 | 175 | 231 | 256 | 269 | 270 | 362 | 381 | 387 | 404 | 463 | 504 | 559 | Haplotype occurence | ||
| Inia spp. | A | T | the | G | C | G | G/A | A | A | T | C | C/T | C/T | C | C/T | A | ? | ? | ? |
| Sotalia fluviatilis | T | T | A | G | C/T | G | C/T | T | C | C/T | C | T | T | C | T | A | ? | ? | ? |
| Sotalia guianensis | C | G/T | A/C | A | C/T | G/A | C/T | C/T | C/T | C/T | C/G | C/T | C/T | C/T | C/T | C/A | 22 | 11 | 1 |
| CR_Hap01 | C | T | A | A | T | A | C | C | C | C | C | T | C | C | T | the | 1 | ? | ? |
| CR_Hap02 | C | T | A | A | T | A | C | T | C | C | C | C | T | C | T | A | 2 | ? | ? |
| CR_Hap03 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | the | 6 | 2 | ? |
| CR_Hap04 | C | T | A | A | T | A | C | T | C | C | C | T | C | C | T | A | 3 | 5 | 1 |
| CR_Hap05 | C | T | A | A | T | A | C | T | C | T | C | T | C | C | T | the | 1 | 2 | ? |
| CR_Hap06 | C | T | A | A | C | the | T | T | T | C | C | T | T | C | T | the | 1 | ? | ? |
| CR_Hap07 | C | T | A | A | T | A | C | T | C | C | C | C | C | C | T | A | 3 | ? | ? |
| CR_Hap08 | C | G | C | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |
| CR_Hap09 | C | T | A | A | T | G | C | T | C | C | C | T | C | C | T | the | 1 | ? | ? |
| CR_Hap10 | C | T | A | A | T | A | C | C | C | C | C | T | T | C | T | the | 1 | ? | ? |
| CR_Hap11 | C | T | A | A | T | A | C | C | C | C | C | C | T | T | T | A | ? | 1 | ? |
| CR_Hap12 | C | T | A | A | T | A | C | C | C | C | C | C | T | C | T | C | ? | 1 | ? |
| CR_Hap13 | C | T | the | A | C | the | C | T | T | C | C | T | T | C | C | A | 1 | ? | ? |
| CR_Hap14 | C | T | A | A | T | A | C | C | C | C | G | C | T | C | T | the | 1 | ? | ? |
| Cytochrome b | |||||||||||||||||||
| adjustable position | 49 | 78 | 124 | 297 | 367 | 396 | 462 | 501 | 528 | 531 | 561 | ||||||||
| Inia spp. | G | T | A | C | G | C | T | T | A | C | T | ? | ? | ? | |||||
| Sotalia fluviatilis | G | C | A | G | G | C | T | the | A | C | T | ? | ? | ? | |||||
| Sotalia guianensis | G/A | C/T | G/A | A | G/A | C/T | C | G | C | T | C | 16 | 7 | 1 | |||||
| CB_Hap01 | G | C | A | A | G | C | C | G | C | T | C | 8 | 4 | 1 | |||||
| CB_Hap02 | A | C | A | the | G | C | C | G | C | T | C | 1 | ? | ? | |||||
| CB_Hap03 | G | T | A | A | G | C | C | G | C | T | C | 1 | ? | ? | |||||
| CB_Hap04 | G | C | G | A | G | T | C | G | C | T | C | 3 | 1 | ? | |||||
| CB_Hap05 | G | C | G | A | A | T | C | G | C | T | C | 3 | 2 | ? | |||||
- Web web web Sites highlighted in bold unambiguously assign the eyeball that is unknown to Sotalia guianensis. Those perhaps not highlighted differentiate haplotypes that are observed. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.
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